Cell penetrating peptides, CCPs, are typically small lysine and/or arginine-rich peptides derived from the transduction domains of a number of proteins, including the third transmembrane domain of the Antennapedia homeodomain (Penetratin) and the HIV-tat protein, which can cross cell membranes without the need for a receptor or other transport carrier. When coupled to these cell permeable peptides, various molecules such as peptides, DNA, and proteins, may be inserted into the cell. A number of standard vectors are available, but you can have any peptide custom made to suit your requirements.
A note of consideration; While a cell permeable peptide such as HIV Tat can cross the cell membrane it does not necessarily mean that it will do so carrying the cargo molecule of interest, let alone into the intended cellular compartment. One may consider adding dyes or other molecules to confirm that the material has been delivered into the cell. Using a scrambled control to rule out nonspecific effects is advisable. Furthermore, it might be necessary to make use of more than one strategy for introducing the molecule.
As with any peptide that is going to be used with cells, it may be worth to consider using acetate instead of TFA, which is the standard counter ion.
Innovagen has serviced the research community since 1992. In that time we have been providing scientists with various CCPs and CCP+cargo complexes, adding to our experience. We know what it is about, and how even the choice of counter ion may very well have an impact on your experiments with cell permeable peptides. We focus on quality and customer service, and this too you will from when turning to us for your requirements.
Examples of references of customers using our custom synthesised cell penetrating peptides include;
H.L. Åmand, H. A. Rydberg, L. H. Fornander, P. Lincoln, B. Nordén, E. K. Esbjörner.
Cell surface binding and uptake of arginine- and lysine-rich penetratin peptides in absence and presence of proteoglycans.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 15 June 2012, ISSN 0005-2736
E. Mattila, H. Marttila, N. Sahlberg, P. Kohonen, S. Tähtinen, P. Halonen, M. Perälä & J. Ivaska.
Inhibition of receptor tyrosine kinase signalling by small molecule agonist of T-cell protein tyrosine phosphatase.
BMC Cancer 2010, 10:7
S. Sandgren, F. Cheng & M. Belting.
Nuclear Targeting of Macromolecular Polyanions by an HIV-Tat Derived Peptide.
J. Biol. Chem. 2002 Vol. 277, No. 41, pp. 38877-38883.
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