Custom service: fusion protein antibody

We provide a custom fusion protein antibody production service. Typically such a project is a rabbit polyclonal anti-peptide antibody, which is what is outlined in the below. However, should your requirements differ from this they can be accommodated. Please contact us for a free consultation in order to determine your requirements as well as project feasibility and estimated cost.

The point mutation specific service package includes;

Design and synthesis immunisation peptide (10 mg, high purity) spanning the fusion point, and the two corresponding control peptides.
Conjugation of the immunisation peptide to KLH
Immunisation of two rabbits using a 12 week standard protocol.
Collection of pre-immune serum and three immune serum samples.
ELISA testing of first serum sample against the immunisation and control peptides.
Collection of specific fusion protein antibodies by affinity purification using a total of 20 ml of crude serum.

Additional services can be added to this package. Some examples include;

Antibody aliquotation
Antibody labelling, such as biotin or FITC.

The projected time line for your custom fusion protein antibody project is as follows;

5 weeks - Peptide design, production and conjugation
6 weeks - First serum sample after primary immunisation
3 weeks - Additional serum sample.
2 weeks - Fusion protein specific affinity purification.

Additional information

Order fusion protein antibodies

References

Examples of references of customers using our custom fusion protein antibody service include;

A. Behboudi, F. Enlund, M. Winnes, Y. Andrén, A. Nordkvist, I. Leivo, E. Flaberg, L. Szekely, A. Mäkitie, R. Grenman, J. Mark & G. Stenman. 2006.
Molecular Classification of Mucoepidermoid Carcinomas—Prognostic Significance of the MECT1–MAML2 Fusion Oncogene
Genes, Chromosomes & Cancer 45:470–481 (2006)

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