Custom service: isoform specific antibody

We provide a custom isoform specific antibody production service. Typically such a project is a rabbit polyclonal anti-peptide antibody, which is what is outlined in the below. However, should your requirements differ from this they can be accommodated. Please contact us for a free consultation in order to determine your requirements as well as project feasibility and estimated cost.

The isoform specific antibody service package includes;

• Design and synthesis of the specific isoform immunisation peptide (10 mg, high purity) and necessary control peptides.
• Conjugation of the immunisation peptide to KLH
• Immunisation of two rabbits using a 12 week standard protocol.
• Collection of pre-immune serum and three immune serum samples.
• ELISA testing of first serum sample against the immunisation peptide and control peptides.
• Isoform specific affinity purification using a total of 20 ml of crude serum.

Additional services can be added to this package. Some examples include;

• Antibody aliquotation
• Antibody labelling, such as biotin or FITC.

The projected time line for your isoform specific polyclonal rabbit antibody project is as follows;

• 4 weeks - Peptide design, production and conjugation
• 6 weeks - First serum sample after primary immunisation
• 3 weeks - Additional serum sample.
• 2 weeks - Isoform specific affinity purification.

Additional information

• Isoform specific antibodies
• Designing synthetic peptides as antigens for antibody production.

Order isoform specific antibodies

• Contact us for a free consultation
• Order custom isoform specific antibody