Synthetic peptides can be labelled with just about any fluorescent dye you like, and since the labelling can be done during synthesis, it is site specific. Another difference compared to labelling proteins and antibodies is that, simply due to the size difference, the amounts of labelling reagent required are much larger. Thus, there is a lot to gain by using comparatively less expensive reagents, such as 5-FAM and TAMRA, while maintaining the high standards of every other aspect of the production.
A note of consideration; When constructing synthetic peptides incorporating dyes, the choice of location for the molecule may have an impact on background signal. Due to the fact that peptides are synthesized from C-terminus to N-terminus, one can ensure that only peptides of full length carry the dye when it is attached to the N-terminus - if an acetylation cycle is run for each amino acid coupling step. Conversely, when the dye is attached to the C-terminus, all peptide molecules will carry the label.
Innovagen has serviced the research community since 1992. Over the years we have been providing synthetic peptides with various fluorescent dyes, and we know how just choosing the right synthesis strategy can make a world of difference for the final product. But our commitment goes beyond this as we believe that quality always pays off and we will go that extra mile for the benefit of your project. Please take a few moments to read about our view on the production of high quality peptides.
Examples of references of customers using our custom peptide synthesis services for the production of labelled peptides, include;
H. L. Åmand, H. A. Rydberg, L. H. Fornander, P. Lincoln, B. Nordén, E. K. Esbjörner
Cell surface binding and uptake of arginine- and lysine-rich penetratin peptides in absence and presence of proteoglycans
BBA November, 2012 Vol. 1818 no. 11 Pages 2669–2678
E. Andersson Nordahl, V. Rydengård, P. Nyberg, D. P. Nitsche, M. Mörgelin, M. Malmsten, L. Björck & A. Schmidtchen.
Activation of the complement system generates antibacterial peptides
PNAS November 30, 2004 vol. 101 no. 48 16879-16884
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